Description
The Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System(a) contains both Rabbit Reticulocyte Lysate and Wheat Germ Extract for comparing in vitro translation systems. Reticulocyte Lysate is prepared from New Zealand white rabbits (1). The Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cellular debris. Both systems contain the cellular components necessary for protein synthesis. The systems have been treated with micrococcal nuclease, which destroys endogenous mRNA and results in minimal background translation.