Exonuclease III
from
Promega
Description
Exonuclease III is a 3´→5´ exonuclease specific for double-stranded DNA. The enzyme catalyzes the stepwise removal of mononucleotides starting from a 3´-OH at nicks, blunt ends, recessed ends and 3´-overhangs of less than 4 bases (1), yielding nucleoside 5´-phosphates. Exonuclease III will also degrade DNA from 3´-phosphate ends due to its intrinsic 3´-phosphatase activity (2). In addition, the enzyme has apurinic endonuclease activity and ribonuclease H activity (2). Exonuclease III is used in conjunction with S1 nuclease for unidirectional deletion of sequences from the termini of DNA fragments (3).
Specifications/Features
Unit Definition:
One unit is defined as the amount of enzyme required to produce 1nmol of acid-soluble nucleotides from double-stranded DNA in 30 minutes at 37°C.