luciferase Assay Kit, Dual-Glo™ from Promega


Product	luciferase Assay Kit, Dual-Glo™
Company	Promega
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Target	Luciferase
Catalog Number	E2940
Method	Luciferase
Original Item Name	Dual-Glo™ Luciferase Assay System
Quantity	100 ml
Sample Type	Mammalian cell culture media
Species	mammalian
Target/Molecule Descriptor	Dual-Glo™

Description

The Dual-Glo™ Luciferase Assay System(a,b,c,d,e) is a homogeneous reagent system that enables fast and simple quantitation of stable luminescence from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. The Dual-Glo™ Luciferase Assay System provides high Z´-factors for cell-based, high-throughput screening applications. With the Dual-Glo™ System, internal controls can be established to minimize sample variability. Internal controls can also be used to reduce false positive and false negative readings caused by nonspecific factors, such as cytoxicity. In the Dual-Glo™ Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli and the activity of the co-transfected control reporter provides an internal control to normalize results. The system is optimized for batch processing both 96- and 384-well plates and is broadly compatible with mammalian cell culture media.

References

Belov, G.A., Altan-Bonnet, N., Kovtunovych, G., Jackson, C.L., Lippincott-Schwartz, J. and Ehrenfeld, E. (2007) Hijacking components of the cellular secretory pathway for replication of poliovirus RNA. J. Virol. 81 , 558–567 .

Ullah, M.S., Davies, A.J. and Halestrap, A.P. (2006) The plasma membrane lactate transporter MCT4, but not MCT1, is up-regulated by hypoxia through a HIF-1alpha-dependent mechanism. J. Biol. Chem. 281 , 9030-9037 .

Campbell, D.B., Sufcliffe, J.S., Ebert, P.J., Militerni, R., Bravaccio, C., Trillo, S., Elia, M., Schneider, C., Melmed, R., Sacco, R., Persico, A.M. and Levitt, P. (2006) A genetic variant that disrupts MET transcription is associated with autism. Proc. Natl. Acad. Sci. U S A 103 , 16834-16839 .

Martins, G.A., Hutchins, A.S. and Reiner, S.L. (2005) Transcriptional activators of helper T cell fate are required for establishment but not maintenance of signature cytokine expression. J. Immunol. 175 , 5981–5985 .

Lin, X., Ruan, X., Anderson, M.G., McDowell, J.A., Kroeger, P.E., Fesik, S.W. and Shen, Y. (2005) siRNA-mediated off-target gene silencing triggered by a 7 nt complementation. Nucleic Acids Res. 33 , 4527-4535 .

Verge´, V., VonLanthenm, M., Masson, J.M., Trachsel, H., and Altmann, M. (2004) Localization of a promoter in the putative internal ribosome entry site of the Saccharomyces cerevisiae TIF4631 gene. RNA 10 , 277–286 .

Chen, Q.Y. and Jackson, N. (2004) Human CD1D gene has TATA boxless dual promoters: An SP1-binding element determines the function of the proximal promoter. J. Immunol. 172 , 5512–5521 .

Shirasawa, S., Harada, H., Furugaki, K., Akamizu, T., Ishikawa, N., Ito, K., Ito, K., Tamai, H., Kuma, K., Kubota, S., Hiratani, H., Tsuchiya, T., Baba, I., Ishikawa, M., Tanaka, M., Sakai, K., Aoki, M., Yamamoto, K. and Sasazuki, T. (2004) SNPs in the promoter of a B cell-specific antisense transcript, SAS-ZFAT, determine susceptibility to autoimmune thyroid disease. Hum. Mol. Genet. 13 , 2221-2231 .

Perkinton, M.S., Standen, C.L., Lau, K.F., Kesavapany, S., Byers, H.L., Ward, M., McLoughlin, D.M. and Miller, C.C. (2004) The c-Abl tyrosine kinase phosphorylates the Fe65 adaptor protein to stimulate Fe65/amyloid precursor protein nuclear signaling. J. Biol. Chem. 279 , 22084-22091 .

Compton, T., Kurt-Jones, E.A., Boehme, K.W., Belko, J., Latz, E., Golenbock, D.T., and Finberg, R.W. (2003) Human Cytomegalovirus activates inflammatory cytokine responses via CD14 and Toll-like Receptor 2. J. Virol. 77 , 4588-4596 .

Hirose, F., Ohshima, N., Kwon, E.-J., Yoshida, H., and Yamaguchi, M. (2002) Drosophila Mi-2 Negatively Regulates dDREF by Inhibiting Its DNA-Binding Activity. Mol. Cell. Biol. 22 , 5182-5193 .