Klenow Fragment, Exonuclease Minus
from
Promega
Description
DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is a DNA-dependent DNA polymerase that lacks both the 5´→3´ and the 3´→5´ exonuclease activities present in intact E. coli DNA Polymerase I (1,2). It is used for random primer labeling (3,4) and in strand displacement amplification (5). Klenow Fragment, Exonuclease Minus, will leave a single-base 3´ overhang on a significant proportion of DNA fragments during fill-in of 5´-overhangs. Therefore, this enzyme is not recommended for preparation of blunt-ended fragments for ligation.
Specifications/Features
Comments:
M2181
Unit Definition:
One unit is defined as the amount of enzyme that incorporates 10nmol of total deoxyribonucleotides into TCA-insoluble material in 30 minutes at 37°C. The reaction conditions are: 67mM potassium phosphate (pH 7.5 at 25°C), 6.7mM MgCl2, 1mM DTT, 50μg/ml activated calf thymus DNA, and 33μM dATP, dCTP, dGTP and dTTP (a mix of unlabeled and [3H]dTTP).