SV Total RNA Isolation System from Promega

Product Specs
ItemSV Total RNA Isolation System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberZ3105
Time1 hr.
Quantity250 preps
SourceBlood, Cell/Tissue
Nucleic Acid TypeTotal RNA
Sample TypeBlood samples, tissue samples, cultured cells

Specifications/Features

Starting Amount:
60mg of tissue

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) miR-19a-3p and miR-19b-3p repress Nurr1 and Nur77 to promote microglial inflammation after spinal cord injuryFrontiers in Cellular NeuroscienceMarch 23, 2026Faezeh Sahebdel, Aliabbas Zia, Hector Ramiro Quintá, Andres Stucky, Leslie R. Morse, Julie K. Olson, Ricardo A. Battaglinoanalysis After incubating the microglia cells for 24 h, we used the SV Total RNA Isolation kit (Promega, #Z3100) to lyse and isolate total RNA. The Advantage for RT-PCR kit (Clontech Laboratories, Takara Bio Company,

(2) Suppressive Effects of an Inhibitor Composition on Skin Ulceration and Transcriptomic Analysis in the Sea Cucumber Apostichopus japonicus Exposed to No. 0 Diesel OilBiology (Basel)March 18, 2026Xiaonan Li, Yajie Deng, Shufeng Li, Haoran Xiao, Fenglin Tian, Qi Ye, Lingshu Han, Chong Zhao, Jun Ding1), and n = 3 biological replicates were analyzed per group. RNA was extracted with Promega’s SV kit (Z3100, Promega, Madison, WI, USA) and treated on-column with DNase I. Expression levels were derived via 2−ΔΔCt

(3) The Overexpression of Myelin and Lymphocyte Protein (MAL) Downregulates MUC1 and Enhances Cisplatin Sensitivity in Non-Small Cell Lung Cancer CellsJournal of CancerMarch 17, 2026Ana Karina Saldaña-Villa, Bismarck Vázquez-Almazán, Catalina Flores-Maldonado, Juan Carlos Vizuet-de-Rueda, Elisa Natalia Oropeza-Durán, José Bonilla-Delgado, Enoc Mariano Cortés-Malagón, Rubén Gerardo Contreras, Luis Manuel Teran, Blanca Ortiz-Quintero, et al.polymerase chain reaction RT-qPCR) analysis The SV Total RNA Isolation System Kit (Promega, Cat. No. Z3100) was used to extract total RNA from all samples. For quantitative real-time PCR (RT-qPCR), RNA was treated

(4) Overcoming phagocytosis resistance of hypervirulent Klebsiella pneumoniae by directly targeting capsulesMicrobial CellFebruary 16, 2026Shogo Tsubaki, Touya Toyomoto, Rika Tanaka, Jin Imai, Juntaro Matsuzaki, Katsuto Hozumi, Hitoshi Tsugawaextracted from K. pneumoniae, using the SV Total RNA Isolation System (Promega, Madison, WI, USA; cat. no. Z3100). Complementary DNA was synthesized with the PrimeScript RT Reagent Kit (TaKaRa, Ohtsu, Japan; cat.

(5) Melatonin Mitigates Central Sensitization and Nociplastic Pain in Spinal Cord and Dorsal Root Ganglia of FM Rat Model: Modulation of SIRT1/PGC-1α/MAPK/NF-κB SignalingJ Neuroimmune PharmacolFebruary 13, 2026Jehad Osama, Amira A. El-Gazar, Ghada M. Ragab, Nesrine S. El-Sayed, Ahmed S. KamelMT2R mRNA in relation to β-actin was determined. Utilizing the SV total RNA isolation system (Cat.#Z3101; Promega, WI, USA), total RNA was extracted and subsequently transformed to cDNA using an Invitrogen

(6) Silymarin Attenuates Arthritis and Myositis in a Murine Model of Acute Infection by Chikungunya and Mayaro VirusesACS Infectious DiseasesFebruary 13, 2026Rafaela Lameira Souza Lima, Ariane Coelho Ferraz, Marília Bueno da Silva Menegatto, Oluwashola Samuel Ola-Olu, Pedro Henrique Guimarães, Giovana Mesquita Oliveira de Castro Domingos, Allen Rene Ruiz Hernández, Maria Eduarda Diniz Starling, Pedro Alves Machado-Junior, Frank Silva Bezerra, et al.cDNA Synthesis Total RNA was extracted from all collected organs using the SV Total RNA Isolation System kit (no. Z3105, Promega, USA), following the manufacturer’s instructions. This kit includes an on-column DNase I treatment,

(7) Study on the Stability of Reference Genes and HSP60 for Expression Analysis in Chilo suppressalis in Response to Humidity StressInsectsJanuary 7, 2026Ming Zhao, Yong Chen, Hai-Bo Zhang, Jian-Fei Mei, Ya-Jun GuoTranscription Total RNA was isolated from each sample using the SV Total RNA isolation system (Promega Z3100, Madison, WI, USA). RNA integrity was analyzed by agarose gel electrophoresis, and then RNA purity and

(8) Integrated application of transcriptomics and metabolomics provides insights into gonadal differentiation in Mesocentrotus nudusScientific ReportsDecember 20, 2025Abudula Abulizi, Weiyi Su, Xiaoxiao Huang, Heng Xiang, Zhihui Sun, Yaqing Changthree undifferentiated testes, for qRT-PCR analysis using the SV Total RNA Isolation System (Promega, Z3100), according to the manufacturer’s instructions. The RNA concentration and purity were measured with

(9) CD38 is a key mediator of NAD+ depletion in the brain of ZIKV-infected miceiScienceDecember 19, 2025Georgia N. Saraiva, Bruna G. Sousa, Nicole M.S. Souza, Louise C. Vitorino, Raquel C. da Silva, Thiago S. Bacelar, Matheus O. Atella, Lorena O. Fernandes-Siqueira, Isis Nem de Oliveira Souza, Eduardo Nunes Chini, et al.assays,Critical commercial assays,Critical commercial assays ,, SV Total RNA Isolation System,Promega,Cat#Z3105 High-Capacity cDNA Reverse Transcription Kit,Applied Biosystems,Cat#4368814 SYBR™ Green Master Mix,Applied Biosystems

(10) Liver Stiffness Characterization of OGT LKO Mouse Model of Progressive Liver FibrosisJournal of Ultrasound in MedicineDecember 4, 2025Gilles Renault, Lenin Chinchilla, Lourdes Massengo, Pierre Monbernard, Fadila Benhamed, Marion Regnier, Raphael Denis, Catherine Postic, Anthony Novell, Jean‐Luc Gennissonpowder was used for RNA extraction using the SV Total RNA Isolation System (Promega, Madison, WI, USA; Z3101). mRNAs were reverse transcribed, and cDNA levels were measured by quantitative PCR (qPCR) (LightCycler480

(11) Modulation of IL-1β and TGF-β1 Gene Expression in Stress-Induced Depression Rat Supplemented with Malaysian Acacia HoneyFoodsNovember 14, 2025Anis Syamimi Mohamed, Hussin Muhammad, Nik Aina Syazana Nik Zainuddin, Nur Liana Md Nasir, Mohd Rahimi Ashraf Abd Rahman, Lau Mei Siu, Abdah Md Akim, Kalavathy Ramasamy, Mizaton Hazizul Hasan, Zolkapli Eshakcaecum and hypothalamus) using the SV Total RNA Isolation System (Promega, Madison, WI, USA, Cat # Z3105). RNA purity and quantity were measured by NanoDrop Lite Spectrophotometer (Thermo Scientific, Waltham,

(12) A conserved mechanism for the retrieval of polyubiquitinated proteins from ciliaCellNovember 13, 2025Sven M. Lange, Jeremy A. Bennett, Robyn J. Eisert, Alan Brownand washed once with 1 mL PBS. Total RNA was isolated using SV Total RNA Isolation System (Promega, #Z3101) following the manufacturer’s instructions, flash frozen in liquid nitrogen and stored at −80 °C until

(13) An RNA sponge directs the transition from feast to famine in Caulobacter crescentusNat CommunOctober 27, 2025Laura N. Vogt, Manuel Velasco Gomariz, Malte Siemers, Kai Papenfort, Kathrin S. Fröhlichthe Hot Phenol method as described previously40 or using the SV Total RNA Isolation System (Promega, #Z3100) following the manufacturer’s instructions. Subsequent Northern blot analysis was performed as described

(14) High-efficiency homology-directed insertion into the genome using the engineered homing endonuclease ARCUSNucleic Acids ResOctober 6, 2025Laura Christian Resly, Alan L Tubbs, Alexander J Vogel, Jo Ann Hux, Ian A MacDonald, Jason Harris, Adam Mischler, Ginger H Tomberlin, Kathryn S Evans, Rhese Thompson, et al.treatment, the RNA was column purified with the SV Total RNA Isolation System (Promega, Madison, WI, Cat# Z3105) normalized to 1.0 μg/μl, aliquoted, and stored at −80°C until ready for use. Cas9 RNA was purchased

(15) Ferulic acid mitigates 3-Nitropropionic acid-induced Huntington's disease via modulation of Nrf2/HO-1, TLR4/NF-κB, and SIRT1/p53 signaling pathwaysFront PharmacolOctober 1, 2025Mohamed A. Abdelgawad, Abdallah M. Gendy, Sameh S. Zaghlool, Wessam H. Elesawy, Mai F. Ragab, Mohamed I. Kotb El-Sayed, Alaadin E. El-Haddad, Hussein S. Mohamed, Izzeddin Alsalahat, Marwa A. Essa1981; Nasser et al., 2022). Briefly, total RNA was extracted using the SV Total RNA system (Cat. No. Z3100, Promega, Madison, WI, United States), and the purity was verified by the OD 260/280 nm absorbance ratios.

(16) Gene body methylation regulates gene expression and mediates phenotypic diversity in natural Arabidopsis populationsNature PlantsSeptember 12, 2025Zaigham Shahzad, Elizabeth Hollwey, Jonathan D. Moore, Jaemyung Choi, Gaëlle Cassin-Ross, Hatem Rouached, Matthew R. Robinson, Daniel Zilbermanper experiment. Total RNA was extracted using the SV Total RNA Isolation System (Promega, cat. no. Z3101). One microgram of total RNA was used for first-strand cDNA synthesis using SuperScript IV Reverse Transcriptase

(17) Parthanatos drives cognitive decline in repeated brain trauma: MSC-derived exosomes as a novel therapeutic strategyFrontiers in PharmacologySeptember 2, 2025Heba Mohammed Refat M. Selim, Amira A. El-Gazar, Dalaal M. Abdallah, Hagar B. Abo-Zalam, Ghada M. Ragab, Ahmed N. Abdallah, Rabab A. El-Gazar, Sultan Alshehri, Einas M. Yousef, Rayan Ballal, et al.determined using the 2−ΔΔCT formula. The SV Total RNA Isolation System (Promega, WI, United States; #Z3101) was employed to extract total RNA, followed by processing the samples with an Invitrogen kit (CA, United

(18) Genotype‐Specific Small EVs Released by Giardia lamblia Act as Mediators of Phenotypic Adaptation Under Metronidazole‐Induced StressJournal of Extracellular VesiclesSeptember 1, 2025Gabriel Luna Pizarro, Jerónimo Laiolo, Nehuén Salas, Rocío G. Patolsky, Luciano Díaz Pérez, Camilo Cotelo, Constanza Feliziani, Andrea Silvana Rópolo, María Carolina TouzThermo Fisher Scientific Inc.). Total RNA was extracted using the SV Total RNA Isolation System (Cat. No. Z3100, Promega, Madison, WI, USA) following the manufacturer's protocol. Two micrograms of total RNA were

(19) IgG autoantibodies in bullous pemphigoid induce a pathogenic MyD88-dependent pro-inflammatory response in keratinocytesNat CommunAugust 6, 2025Lei Bao, Christian F. Guerrero-Juarez, Jing Li, Manuela Pigors, Shirin Emtenani, Yingzi Liu, Adrian P. Mansini, Yulu F. Wang, Aadil Ahmed, Norito Ishii, et al.processed using the Promega SV Total RNA Isolation System (Promega, Fitchburg, Wisconsin, USA; Cat# Z3105) per manufacturer instructions. Samples were run as duplicates (skin) or triplicates (keratinocytes). RT-PCR RT-PCR

(20) ALS-associated RNA-binding proteins promote UNC13A transcription through REST downregulationThe EMBO JournalJuly 24, 2025Yasuaki Watanabe, Naoki Suzuki, Tadashi Nakagawa, Masaki Hosogane, Tetsuya Akiyama, Naotoshi Kageyama, Yukino Funayama, Hitoshi Warita, Satoru Morimoto, Hideyuki Okano, et al.Kit,Invitrogen,C10365 SimpleChIP Enzymatic Chromatin IP Kit,Cell Signaling,9003S SV Total RNA isolation system,Promega,Z3100 PrimeScript RT reagent kit,Takara Bio,RR037A SuperScript VILO cDNA Synthesis Kit,Thermo Fisher,11754050 Fast

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SV Total RNA Isolation System From Promega
The SV Total RNA Isolation System was designed to purify intact total RNA from tissues, cultured ... Read More
SV Total RNA Isolation System From Promega
It has always been a hassle to obtain pure RNA from tissue or cultured cells. RNA is frequently ... Read More