Mouse Anti- Human CD163 Monoclonal Antibody, Unconjugated
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United States Biological
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Product
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Mouse Anti- Human CD163 Monoclonal Antibody, Unconjugated
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Company
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United States Biological
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Price
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Pricing Info
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More Information
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View Company Product Page
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Target
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CD163
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Catalog Number
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C2548-90D2
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Quantity
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100ug
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Species
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Human
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Reactivity
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Human
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Conjugate/Tag/Label
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Unconjugated
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Applications
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E FC IHC IP WB
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Host
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Mouse
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Type
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Monoclonal Antibody
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Isotype
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IgG1
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Original Item Name
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CD163 (Hemoglobin/Haptoglobin Complex Receptor, M130 antigen, Ber-Mac3, Ki-M8, SM4)
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Target/Molecule Synonym
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(Hemoglobin / Haptoglobin Complex Receptor, M130 antigen, Ber-Mac3, Ki-M8, SM4)
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Description
CD163 is a membrane glycoprotein of 130kD, which is expressed exclusively on the cell surface of human monocytes and macrophages that predominantly evolve in the late phase of inflammation. The CD163 is a member of the scavenger receptor cysteine-rich superfamily. CD163 is a scavenger receptor for the hemoglobin-haptoglobin complex. The protein expression is markedly induced by glucocorticoids while it is down-regulated by cyclosporin A and by phorbol esters. CD163 is sensitive to ionic detergents and to chloroform/methanol treatment. Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a main population of macrophages in the placenta, varying degrees of macrophages in inflamed tissue, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis; in alveolar macrophages and in Kupffer cells a double staining can be observed with this clone, which is not the case in other tissues.
Applications:
Suitable for use in Western Blot, Flow Cytometry, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:10
Flow Cytometry: 1:10
Immunohistochemistry (Frozen and paraffin sections): 1:10
Optimal dilutions to be determined by the researcher.
Note:
C2548-90D2 is sensitive to ionic detergents and to chloroform/methanol treatment.
Storage and Stability:
May be stored at 4¡C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20¡C. Aliquots are stable for at least 12 months at -20¡C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.