SPIN-Column Low Density NICKEL, 25 x 100 µl, $179
from
Gold Biotechnology
Description
Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.
Affinity Chromatography is the purification technique which is widely used. It is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.
IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with a divalent metal (Ni2+, Cu2+, Zn2+ or Co2+). The resulting resin (ready to use) is ideal for rapid purifications of His-tagged proteins.