Description
The integration of 2D polyacrylamide gel electrophoresis (2D-PAGE) with biological mass spectrometry is widely regarded as a core technology platform for proteomic analysis. 2D-PAGE gels offer unparalleled resolving power for complex samples limited only in the dynamic range and ability to resolve small and large proteins. Quantitative image based analysis of separated proteins has been a major development in biomedical investigations.Shimadzu Phoretix 2D expression software is the advanced platform for gel analysis.
Features in addition to the core platform - Shimadzu Phoretix 2D software
- Sophisticated spot detection and quantitation
Enhanced spot detection algorithms designed to increase the precision and accuracy for optimal detection. The advanced nature of the algorithm also supports the suppression of major noise features in spot detection and quantitation, even with low signal intensities. - Increased automation
To increase the throughput of analysis without compromising the quality of the data automated routines support advanced spot detection, spot splitting, background suppression and subtraction, gel averaging, volume calculation, matching methods, addition of spots to a reference gel and calibrates all matched gels. - Complete editing software tool sets
Designed to maximize the flexibility of spot detection and reporting. Advanced tools to simplify gel comparisons and to take into account gel warping.