QCM Collagen Cell Invasion Assay, 96-well (8 µm), fluorimetric
from
EMD Millipore
|
|
|
|
Product
|
QCM Collagen Cell Invasion Assay, 96-well (8 µm), fluorimetric
|
|
Company
|
EMD Millipore
|
|
Price
|
|
|
More Information
|
View Company Product Page
|
|
Detection
|
Fluorescent
|
|
Reactivity
|
Vertebrates
|
|
Source/Expression System
|
Cytology test kits or supplies
|
|
Target
|
Cell Invasion
|
|
Catalog Number
|
ECM556
|
|
Method
|
Fluorometric (CyQuant)
|
|
Original Item Name
|
QCM Collagen Cell Invasion Assay, 96-well (8 µm), fluorimetric
|
|
Quantity
|
1 plate
|
|
Sample Type
|
Cells
|
|
Target/Molecule Descriptor
|
QCM™
|
|
|
|
Description
QCM Collagen Cell Invasion Assay, 96-well (8 µm), fluorimetric Cell Migration / Invasion. Introduction Penetration of the subendothelial basement membrane marks a critical turning point in the metastatic process. As proliferating neoplastic cells attempt to escape the primary tumor site, local invasion of the surrounding tissue (interstitial stroma) must occur. Neovascularization is initiated by expression of angiogenic factors (e.g. FGF, VEGF, HGF), providing nutritional requirements and access to the vascular system. Prior to penetrating the blood vessel endothelium and gaining access to the blood stream (intravasation), cancer cells must invade local tissues by degrading ECM components and ultimately, transverse the basement membrane. Once in circulation, these cells can form metastatic colonies at secondary locations, making this membrane a key invasive barrier. The basement membrane surrounding the blood vessel endothelium is a thin, specialized network of extracellular matrix proteins (ECM) that serves many functions. Comprised of proteins and proteoglycans, such as collagen, laminin, entactin, fibronectin, heparin sulfate and perlecan, this membrane acts as a physical barrier between the epithelium and underlying tissues. It provides cell surface anchorage (via integrins, receptor kinases, and cell surface proteoglycans), induces cellular differentiation, gives architectural support, and limits the migration of normal cells. The ability of tumor cells to degrade the ECM components of the basement membrane and surrounding tissues is directly correlated with metastatic potential. By releasing proteolytic enzymes (e.g. MMP collagenases, plasminogen activators, cathepsins), cancer cells are able to breach the membrane and penetrate the blood vessel wall (1). Collagen, the primary structural element of the basement membrane and tissue scaffolding protein, represents the main deterrent in the migration of tumor cells. The ability to study cell invasion through a collagen barrier, is of vital importance for developing possible metastatic inhibitors and therapeutics. The new CHEMICON QCM™ 96-well Collagen-based Invasion Assay (ECM556) provides an efficient, in vitro system for quantitative, high-throughput analysis of tumor cell invasion. In the QCM™ 96-well Collagen-based Invasion Assay (ECM556), invaded cells on the bottom of the insert membrane are dissociated from the membrane when incubated with a specially formulated Cell Detachment Buffer. The invasive cells are subsequently lysed and detected by the patented CyQuant dye (Molecular Probes) (2-3). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (4). The CHEMICON QCM™ 96-well Collagen-based Invasion Assay (ECM556) eliminates cell pre-labeling, fixing/staining, swabbing, and manual counting. The 96-well insert and homogenous fluorescence detection format allows for high-throughput, quantitative comparison of multiple samples. In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
liQCM™ 8µm 96-well Chemotaxis Cell Migration Assay (ECM510)
liQCM™ 5µm 96-well Chemotaxis Cell Migration Assay (ECM512)
liQCM™ 3µm 96-well Chemotaxis Cell Migration Assay (ECM515)
liQCM™ 96-well Cell Invasion Assay (ECM555)
li24-well Insert Cell Migration and Invasion Assay Systems
liCytoMatrix™ Cell Adhesion strips (ECM protein coated)
liQuantiMatrix™ ECM protein ELISA kits Test Principle The CHEMICON Cell Invasion Assay is performed in a 96-well invasion plate based on the Boyden chamber principle. This plate contains 96 inserts; each insert contains an 8 µm pore size polycarbonate membrane coated with a thin layer of polymerized collagen. The collagen layer occ
Specifications/Features
Sterile 96-well Cell Invasion Plate Assembly: (Part No. 90249) One 96-well feeder tray, and one 96-well Cell Invasion Chamber plate with collagen-coated inserts.;96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.;Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.;4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.;CyQuant GR Dye®1: (Part No. 90132) One vial - 75 mL