Blood Vessel Staining Kit, Alkaline Phosphatase conjugated
from
EMD Millipore
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Product
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Blood Vessel Staining Kit, Alkaline Phosphatase conjugated
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Company
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EMD Millipore
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Price
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More Information
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View Company Product Page
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Applications
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Immunohistochemistry;Immunohistochemistry (Paraffin)
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Detection
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Chromogenic
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Method
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IHC staining
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Original Item Name
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Blood Vessel Staining Kit, Alkaline Phosphatase conjugated
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Reactivity
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Human
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Source/Expression System
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Histology reagents or solutions or stains
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Target
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Blood Vessels (CD31 / Von Willebrand Factor (vWF))
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Catalog Number
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ECM595
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Quantity
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1 kit
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Description
Blood Vessel Staining Kit, Alkaline Phosphatase conjugated Reagents and materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections and when stored at 2-8ºC is stable up to the expiration date. Background: Angiogenesis and tumor-associated neovascularization are complex processes by which new blood vessels are formed from pre-existing vasculature. Several studies implicate tumor-associated blood vessel formation as a central part in the process of growth, invasion and metastasis of malignancies (Rak et al., 1995; Jahroudi & Greenberger, 1995; Saphir, 1997). The most widely used endothelial cell markers for studying angiogenesis/neovascularization are von Willebrand Factor (Factor VIII Related Antigen) and CD31 (PECAM-1). von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. CHEMICONs von Willebrand Factor antibody reacts specifically with the endothelial cells of blood vessels and is a useful marker for the identification of endothelial lineage in tumors. It is important to note that not all endothelial cells synthesize von Willebrand factor, therefore a small percentage of these vascular-rich tumors might not stain with this antibody. CD31 or platelet endothelial adhesion molecule (PECAM-1), is a type I integral membrane glycoprotein and a member of the immunoglobulin super family of cell surface receptors. It is strongly expressed by all endothelial cells and weakly by several types of leucocytes (Parums et al., 1990). According to several studies, staining of blood vessels with CD31 antibodies has shown to be suitable for the identification of angiogenesis in several types of malignancies such as lung (Giatromanolaki et al., 1996), breast (Horak et al., 1992) and colorectal cancer (Takebayashi et al., 1996). CHEMICONs monoclonal antibody to CD31 is very specific for the staining of vascular endothelial cells in normal and cancerous tissue. CHEMICONs Blood Vessel Staining Kit is a very sensitive immunohistochemical tool for the detection of vascular endothelial cells, containing both anti-von Willebrand Factor and anti-CD31 primary antibodies and is applicable to a variety of species. This AP kit provides a simple, all-in-one method for the staining of frozen and paraffin-embedded sections in human (both CD31 and vWF) or rat and mouse (vWF only) tissues
Specifications/Features
Rabbit anti-vWF Polyclonal Antibody (Part No. 21540): One vial containing 100 µg at 1 mg/mL.;Mouse anti-CD31 Monoclonal Antibody (Part No. 90214): One vial containing 100 µL at 15 mg/mL (total protein including IgG and Carrier protein).;Goat anti-Rabbit Secondary Antibody (Part No. 21537): One 15 mL ready-to-use solution containing biotinylated goat anti-rabbit IgG in PBS with a carrier protein.;Goat anti-Mouse Secondary Antibody (Part No. 21538): One 15 mL ready-to-use solution containing biotinylated goat anti-mouse IgG in PBS with a carrier protein.;Blocking Reagent (Part No. 20783): One 15 mL ready-to-use solution containing normal goat serum in PBS with a carrier protein.;Streptavidin-AP (Part No. 90215): One 15 mL ready-to-use solution of Streptavidin-AP diluted in Tris-buffered saline (TBS).;Fast Red Solution A (Part No. 90216): Fast Red Violet diluted in TBS. One 15 mL ready-to-use solution.;Fast Red Solution B (Part No: 90217): Naphthol AS Phosphate. One 15 mL bottle ready-to-use solution.;20X Rinse Buffer (Part No. 90218): One 275 mL bottle of 20X PBS.