ImmunoPure ABC Standard Peroxidase Staining Kit
from
Thermo Scientific Pierce Protein Research Products
Description
ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Highly diluted primary antibodies can be used with Pierce Staining Kits, producing comparable stain intensity to other methods that require higher concentrations of antibody.
Two types of enzymatic ABC staining kits are available: alkaline phosphatase and horseradish peroxidase. The kits can come with, or without (Standard Kit) a biotinylated secondary antibody. Kits with a secondary antibody are selected by the species of primary antibody to be used. For example, if the primary antibody (IgG) is produced in mice, the kit selected to detect this antibody should be the ABC Mouse IgG Kit. All you need to create a sensitive detection system is your specific primary antibody, an ABC Kit with biotinylated antibody, and an enzyme substrate.
A Standard Kit includes only the avidin and biotinylated enzyme. It is useful if an ABC Kit is not available for your specific species of primary antibody – or if the specificity of the antibody in the kit is not appropriate for the application. The biotinylated antibody for your specific application can then be purchased separately, along with blocking serum, and used with a standard kit.
The Ultra-Sensitive ABC Peroxidase Staining Kits are more sensitive than the ABC Peroxidase Staining Kits, without exhibiting increased background staining. These kits supply the extra sensitivity needed for localizing antigens present in very small quantities. An expensive primary antibody may be diluted approximately five-fold higher than with the ABC Peroxidase Kit – while producing equal staining intensity.
Systems that offer highly sensitive and rapid detection.