Immobilized NeutrAvidin on Agarose
from
Thermo Scientific Pierce Protein Research Products
Description
When nonspecific binding is a problem in your application, Pierce offers a variety of immobilized NeutrAvidin products as superior alternatives to avidin or streptavidin. NeutrAvidin is a modified avidin derivative that combines several key features to provide a biotin-binding protein with exceptionally low nonspecific binding properties. NeutrAvidin does not contain carbohydrate and has an ideal isoelectric point for the best utility in a variety of applications. For assay development, Pierce has coated NeutrAvidin onto microplates and pre-blocked the plastic surface. Any biotinylated compound can now be attached to the plate and analyzed further. Proteins will retain their structural integrity when bound to a protein instead of bound directly to a plastic surface. The plastic surface may distort the structure of the proteins and prevent accurate detection of the ligand in the assay system. Binding biotinylated compounds to NeutrAvidin provides a stable anchor to the plastic surface.
Applications
- Immunoprecipitation
- Binding Biotinylated DNA to coated plates
- Purifying proteins that bind to biotinylated ligands
Features
- Carbohydrate Free - Just like streptavidin, NeutrAvidin has no carbohydrate, eliminating nonspecific binding problems due to sugars.
- No interaction with cell surface molecules - absence of the Arg-Tyr-Asp sequence (present in streptavidin), which mimics the universal cell surface recognition sequence present in a variety of molecules, eliminates cross-reactivity of cell surface molecules.
- Easily conjugated - Most modified avidins have lysines that are unavailable for coupling. NeutrAvidin's unique preparation preserves lysines, making conjugations easier.
- Neutral pI - With a pI of 6.3, NeutrAvidin's pI is closer to neutrality than avidin or streptavidin. This eliminates electrostatic interaction which contributes to nonspecific binding.
Less nonspecific binding produces better results and better yields.