QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric
from
EMD Millipore
|
|
|
|
Product
|
QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric
|
|
Company
|
EMD Millipore
|
|
Price
|
|
|
More Information
|
View Company Product Page
|
|
Applications
|
Activity Assay
|
|
Detection
|
Fluorescent
|
|
Reactivity
|
All
|
|
Source/Expression System
|
Cytology test kits or supplies
|
|
Target
|
Cell Migration
|
|
Catalog Number
|
ECM509
|
|
Method
|
Fluorometric (CyQuant)
|
|
Original Item Name
|
QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric
|
|
Quantity
|
1 plate
|
|
Sample Type
|
Cells
|
|
Target/Molecule Descriptor
|
QCM™
|
|
|
|
Description
QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), fluorimetric Cell Migration / Invasion. Introduction Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-migrated cells on the top side of insert, manual staining and counting. Recently a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable. The CHEMICON® QCM™ 24-well Cell Migration Assay does not require cell labeling, scraping, washing or counting. The 24-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples. In the CHEMICON® QCM™ 24-well Migration Assay, invaded cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer. These cells are subsequently lysed and detected by the patented CyQUANTâ GR dye (Molecular Probes) (1-2). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (3). The CHEMICON® QCM™ 24-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells. The CHEMICON® QCM™ 24-well Migration Assay utilizes an 8 mm pore size, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis. In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including: · QCM™ 8µm 96-well Chemotaxis Cell Migration Assay (ECM510) · QCM™ 5µm 96-well Chemotaxis Cell Migration Assay (ECM512) · QCM™ 3µm 96-well Chemotaxis Cell Migration Assay (ECM515) · QCM™ 96-well Cell Invasion Assay (ECM555) · QCM™ 96-well Collagen-based Cell Invasion Assay (ECM556) · 24-well Insert Cell Migration and Invasion Assay Systems · CytoMatrix™ Cell Adhesion strips (ECM protein coated) · QuantiMatrix™ ECM protein ELISA kits Test Principle The CHEMICON® QCM™ 24-well Cell Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert utilizes an 8 mm pore size polycarbonate membrane, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.
Specifications/Features
Sterile 24-well Cell Migration Plate Assembly: (Part No. 90333) Two 24-well plates with 12 inserts per plate (24 inserts total/kit).;Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.;4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.;CyQUANTâ GR Dye1: (Part No. 90132) One vial - 75 µL;Forceps: (Part No. 10203) One each.