2D Micro HPLC System for Proteome Analysis
from
Shimadzu
Description
This high-performance 2D capillary-to-microflow HPLC system co-developed by Shimadzu and the NIH performs separation of complex peptide mixtures with a level of sensitivity and resolution that meets or exceeds present 2D gelelectrophoresis. Combining cation exchange chromatography in the first dimension with capillary reversed-phase chromatography in the second dimension, and using a trap column that allows for various separation conditions to be optimized independently, the system can analyze large numbers of trace peptides from digests. • It is possible to analyze proteins that cannot be analyzed with 2D gel electrophoresis • Automatic analysis with high resolution and sensitivity is possible for proteins with low expression levels • Stable analysis is ensured by on-line sample trapping and automatic trapping and automatic desalination followed by RPHPLC It has proved to be an extremely effective tool for the discovery of unknown proteins with low expression levels. When combined with a high-performance mass spectrometer with an electrospray ionization interface (ESI), unequalled performance and sensitivity can be achieved.