PhosphoSite Detector™
from
JPT Peptide Technologies
Description
Each PhosphoSite-Detector™ represents a single peptide microarray on a glass slide containing an overlapping peptide scan through a protein in triplicates (three identical subarrays ensuring data quality). Subsequent to incubation with target kinase in the presence of radioactive ATP, incorporated phosphate can be detected by autoradiography or phosphorimaging. Alternatively, phospho-specific antibody-based fluorescence readout is applicable for phosphopeptide detection.