Tyrosine Kinase Fluorescence Polarization (FP) Assay Kit, PolarScreen™
from
Invitrogen
Description
Far-Red PolarScreen™ FP Kinase Assay Kits use a far-red flourophore, developed in collaboration with Molecular Probes, as a detection tool to reduce interference when screening compounds that have background fluorescent properties. Assays are read using an excitation wavelength of 610 nm (bandpass 20) and an emission wavelength of 670 nm (bandpass 40) as compared to Green FP Kinase Assay Kits, which use excitation wavelength of 485 nm and 530 nm. As a result, picogram to nanogram quantities of kinase will phosphorylate sufficient peptide substrate to affect an assay window of 125 to 250 mp. PolarScreen™ Far-Red FP Kinase Assay Kits provide the following benefits:
- Reduced compound interference—a novel far-red fluorophore reduces effects of autofluorescent compounds, or from light scatter due to precipitation of compounds, leading to fewer false hits
- Robust—Z´ -factor >0.6 for all assays; high polarization shift
- Easy to use—Pipette, incubate, and measure
- Accurate—Measure true equilibrium binding
- Miniaturizable—Kits are easily adapted for use in 1536-well plates