Akt Western blot Assay Kit
from
EMD Millipore
|
|
|
|
Product
|
Akt Western blot Assay Kit
|
|
Company
|
EMD Millipore
|
|
Price
|
|
|
More Information
|
View Company Product Page
|
|
Detection
|
Request Info
|
|
Form
|
Immunoblot
|
|
Method
|
Immunoblot
|
|
Original Item Name
|
Akt Activity Assay Kit
|
|
Sample Type
|
Cell lysates, tissue extracts
|
|
Target
|
Akt
|
|
Time
|
4 h
|
|
Catalog Number
|
124007-1KIT
|
|
Quantity
|
40 Tests
|
|
|
|
Description
Protein Kinase B Activity Assay KitAVOID FREEZE/THAW CYCLES. Useful for the non-radioactive assay of Akt activity in cell lysate or tissue extract lysates. Akt is first enriched via immunoprecipitation with an anti-Akt antibody and then tested for its ability to phosphorylate GSK-3α, an Akt substrate. Phosphorylated GSK-3α is detected through immunoblotting with anti-GSK-3α phospho-specific antibody. Physiologically, GSK-3α is inactivated by Akt phosphorylation. Akt is activated by diverse stimuli such as growth factors and regulates many cellular processes such as apoptosis, metabolism, and proliferation through interactions with a variety of substrates.
References
Nickolson, K.M., and Anderson, N.G. 2002. Cell Signal. 14, 381.van Weeren, P.C., et al. 1998. J. Biol. Chem. 273, 13150.
Specifications/Features
Kit Contents:
Useful for the non-radioactive assay of Akt activity in cell lysate or tissue extract lysates. Akt is first enriched via immunoprecipitation with an anti-Akt antibody and then tested for its ability to phosphorylate GSK-3α, an Akt substrate. Phosphorylated GSK-3α is detected through immunoblotting with anti-GSK-3α phospho-specific antibody. Physiologically, GSK-3α is inactivated by Akt phosphorylation. Akt is activated by diverse stimuli such as growth factors and regulates many cellular processes such as apoptosis, metabolism, and proliferation through interactions with a variety of substrates.
AKT1
(AKT1)
PTK2B
(Focal adhesion kinase 2)