ApoBrdU-IHC DNA Fragmentation Assay Kit
from
BioVision
Description
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVisions Apo-BrdU-IHCTM Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, Hâ‚‚Oâ‚‚/Urea tablets for color generation and methyl green solution for counter staining the cells., · Control Slides-pos/neg · Blocking Buffer · Hâ‚‚Oâ‚‚/Urea Tablets · Protease K · DAB Tablets · TdT Enzymes · Br-dUTP · 200X Conjugate · 5X Reaction Buffer · Anti-BrdU-Biotin mAb · Methyl Green
Specifications/Features
· Simple procedure; convenient · The assay is sensitive and stable · The assay offers an one-step labeling of apoptotic cells., · Sample type- Tissue sections and fixed cell preparations · Species reactivity- Mammalian · Applications- The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation