Akt Western blot Assay Kit
from
BioVision
Description
Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis. The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate. Phosphorylation of the GSK-3α can be analyzed by Western blot analysis using the phospho-GSK-3α specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected., · Kinase Extraction Buffer · Akt Specific Antibody · Protein A Sepharose · GSK-3α Protein/ATP Mixture · Kinase Assay Buffer · Phospho-GSK-3α Specific Antibody
Specifications/Features
· Simple procedure · The kit is suitable for both research and drug discovery · The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected., · Detection method- Western blot analysis using anti-Phospho-GSK-3α (Ser 21) antibody at 1:1000 dilutions · Sample type- Cell and Tissue lysates · Species reactivity- Mammalian · Applications- The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate.
AKT1
(AKT1)
PTK2B
(Focal adhesion kinase 2)