Description
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.
Provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells flow cytometry.
The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.