Protein A, Qdot 605 conjugated/tagged
from
Quantum Dot Corporation
Description
Useful in a Wide Range of Applications
High affinity binding of protein A to target IgGs combined with the benefits of Qdot® nanocrystals technology, offers significant performance advantages in applications such as:
- Solid-Phase Immunosorbent Assays
- Microarray detection
- Fluorescence in situ Hybridization
- Western Blot
- Simplified multicolor detection when used with Qdot Streptavidin Conjugates
Protein A Characteristics
Originally isolated from Staphylococcus aureus, protein A contains five highly homologous IgG binding domains, each made up of approximately 60 amino acids. The protein has an isoelectric point of approximately 5.1 and it is stable in the pH range of 1 to 12. The binding site for protein A is located within the Fc portion of immunoglobulin molecules.
The binding affinities have been found to vary significantly among IgG molecules of various species and subclasses. For example, protein A binds strongly with rabbit, pig, cat, dog, human IgG1, IgG2, IgG4, IgM, IgA, IgE, and most mouse IgG. However, mouse IgG1 molecules have weak binding affinities, and most rat, sheep, horse and goat IgGs bind weakly, while chicken immunoglobulins do not bind to protein A.
Same optical properties as our other Qdot Conjugates
- Flexible excitation with varied light sources (lamp- or laser-based)
- Extremely bright signal gives improved sensitivity and quantitation
- Excellent photostability allows sufficient of time for imaging and analysis
- Narrow emission allows simple multiplexed detection with color filters
- Simple, efficient detection by eye or with many detection systems
Components
Each kit is supplied with 250µl of the Qdot Protein A Conjugate, 30ml of a specially formulated Qdot Incubation Buffer and a User Manual that includes a protocol for using the product in immunohistochemistry applications using fluorescence microscopy. At conventional working concentrations, approximately 100 staining experiments may be performed.