Two-Step RT-PCR Kit
from
Affymetrix / USB
Description
The Two-Step RT-PCR Kit is designed for flexibility and versatility in carrying out RT-PCR in one or two-tube formats.
- Qualitative analysis of gene expression
- Highly detailed analysis of RNA splice variants
- Optimization of PCR independent of RT
- Analysis of the expression of multiple genes in individual RNA samples with oligo dT primers
Reverse transcription-polymerase chain reaction, or RT-PCR, is a method for converting and amplifying a single-stranded RNA template to yield abundant double-stranded complementary DNA (cDNA) product (1,2). In the RT step, the RT enzyme reverse transcribes an RNA template, yielding single-stranded cDNA. In the PCR step, a thermostable DNA polymerase amplifies the single-stranded cDNA to yield double-stranded cDNA product. RT-PCR may be carried out in several ways. One approach, often termed two-step RT-PCR, involves carrying out the RT (or cDNA synthesis) step in one tube and the PCR step in another tube(1) with gene specific primers, for detection of a single target from a single RNA sample. Another approach involves using one RT reaction, primed with oligo dT, for detection of multiple targets from the same RNA sample. A third approach, often termed one-step RT-PCR involves setting up the RT and PCR simultaneously with gene specific primers and carrying out the reactions sequentially in one tube(2). This is particularly useful for detection of one or a few targets in multiple RNA samples. The USB Two-Step RT-PCR Kit has been designed for use in all of these types of RT-PCR.
Specifications/Features
Kit Contents:
M-MLV Reverse Transcriptase, Taq DNA Polymerase, RT Reaction Buffer (5X) (includes MgCl2), PCR Reaction Buffer (10X) (includes MgCl2), PCR Nucleotide Mix, Ultrapure: 10mM each dATP, dCTP, dGTP, dTTP, Ribonuclease Inhibitor, Recombinant (4 units/µl), Magnesium Chloride (25mM), RNase-Free (DEPC-treated) Water
Comments:
78355 1 KT