Description
The Two-Step RT-PCR Kit is well suited for diverse RT-PCR applications. The two-step/gene specific primer format is excellent for qualitative analysis of gene expression, highly detailed analysis of RNA splice variants, and optimization of PCR independent of RT. The two-step/oligo dT primer format is excellent for analysis of the expression of multiple genes in individual RNA samples. And the one-step format is excellent for rapid analysis of single genes in multiple RNA samples. Depending on the scale of experiments and the size of PCR products, use of the One-Step RT-PCR Kit or the RT Script Kit coupled with the Taq PCR Master Mix or Taq PCR Kit may be more efficient. The Two-Step RT-PCR Kit is one kit with many applications.