One-Step RT-PCR Kit
from
Affymetrix / USB
Description
The One-Step RT-PCR Kit is designed for simplicity and convenience in carrying out RT-PCR in a one-tube format.
Qualitative analysis of expression of one or a few genes in multiple RNA samples
Analysis of specific RNA splice variants
Streamlining the optimization of RT and PCR steps simultaneously
A starting point for analysis of new RNA targets, given that many targets can be amplified successfully without need for optimization
Reverse transcription-polymerase chain reaction, or RT-PCR, is a method for converting and amplifying a single-stranded RNA template to yield abundant double-stranded complementary DNA (cDNA) product (1, 2). In the RT step, the RT enzyme reverse transcribes an RNA template, yielding single-stranded cDNA. In the PCR step, a thermostable DNA polymerase amplifies the single-stranded cDNA to yield double-stranded cDNA product. One step RT-PCR is a variation on the RT-PCR in which all reaction components are mixed in one tube prior to starting the reactions(2). This approach offers simplicity and convenience and minimizes the possibility for contamination.
Specifications/Features
Comments:
78350 1 KT
Kit Contents:
RT-PCR Enzyme Mix, RT-PCR Reaction Buffer, 5X (includes MgCl2), Ultrapure PCR Nucleotide Mix: 10mM each dATP, dCTP, dGTP, dTTP, Ribonuclease Inhibitor, Recombinant (4 units/µl), Magnesium Chloride, 25mM, RNase-Free (DEPC treated) Water