Amylase Assay Kit
from
BioVision
Description
· Amylase Assay Buffer · Amylase Substrate Mix · Amylase Positive Control · Nitrophenol standard (2 mM), Amylases are enzymes that break starch down to sugar molecules. α-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (bakers yeast for instance). α-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. Increased enzyme levels in humans are associated with salivary trauma; mumps due to inflammation of the salivary glands, pancreatitis and renal failure. A simple, direct and automation-ready procedure for measuring α-amylase activity is, therefore, very desirable. BioVisions α-amylase Assay uses ethylidene-pNP-G7 as the substrate. Once the substrate has been specifically cleaved by α-amylase, the smaller fragments produced can be acted upon by α-glucosidase, which causes the ultimate release of the chromophore that can then be measured at 405 nm. The assay can detect α-amylase content as low as 0.2 mU.
Specifications/Features
· Simple procedure; takes ~ less than 40 minutes · Fast and convenient · Sensitive assays for measuring Amylase activity in various biological samples., · Detection method- Absorbance (405 nm) · Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples etc. · Species reactivity- Mammalian · Application- The assay can detect α-amylase content as low as 0.2 mU.