pCL-Eco packaging vector
from
IMGENEX
Description
The pCL-ECO packaging vector is a part of the
RetroMax expression system (Cat#10040K and
10041K) and has been designed to maximize
recombinant–retrovirus titers in a simple, efficient,
and flexible experimental system. By introducing a
retroviral vector into a sell expressing retroviral
proteins, retroviral particles (virions) are shed into the
culture medium at the rate of about 1 infectious
particle/cell/day. Retrovirus tropism is determined
at 3 levels. The first is simply a function of viral
envelope protein, gp70. The envelope determines
which cells the virus will enter. gp70 comes in
three different flavors for gene therapy.
Retroviruses obtained by cotransfection with
pCL-Eco vector will infect mouse and rat cells,
but not human cells.
Specifications/Features
Vector Size:
12.6 kb
Kit Contents:
10 µg in 20 µl 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)