pCL-Ampho packaging vector
from
IMGENEX
Description
The pCL-Ampho packaging vector is a part of the
RetroMax expression system (Cat#10040K) and has
been designed to maximize recombinant–retrovirus
titers in a simple, efficient, and flexible experimental
system. By introducing a retroviral vector into a cell
expressing retroviral proteins, retroviral particles
(virions) are shed into the culture medium at the rate
of 1 infectious particle/cell/day. Retrovirus tropism
is determined at three levels. The first is simply a
function of viral envelope protein, gp70. The
envelope determines which cells the virus will
enter. gp70 comes in three different flavors for
gene therapy. The plasmid DNA can be amplified
by transforming bacteria and growing them on
LB/ampicillin (100 µg/ml) media.
Specifications/Features
Kit Contents:
10 µg in 20 µl sterile water