pCL-10A1 packaging vector
from
IMGENEX
Description
The pCL-10A1 packaging vector is a part of the
RetroMax expression system (Cat#10040K and
10041K) and has been designed to maximize
recombinant–retrovirus titers in a simple, efficient,
and flexible experimental system. By introducing a
retroviral vector into a sell expressing retroviral
proteins, retroviral particles (virions) are shed into the
culture medium at the rate of at the rate of about 1
infectious particle/cell/day Retrovirus tropism is
determined at 3 levels. The first is simply a
function of viral envelope protein, gp70. The
envelope determines which cells the virus will
enter. pCL-10A1 expresses 10A1 envelope
protein that was isolated from a mouse infected
with amphotropic MuLV (2,3). Retroviruses
obtained by cotransfection with pCL-10A1 vector
will infect most mammalian cells including
hamster cells.
Specifications/Features
Kit Contents:
10 µg in 20 µl 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)