pCLNCX Expression Vector
from
IMGENEX
Description
The pCLNCX expression vector is a part of the
RetroMax expression system (Cat#10040K and
10041K) and has been designed to maximize
recombinant–retrovirus titers in a simple, efficient,
and flexible experimental system. All members of the
RetroMax expression vector family (pCLXSN,
pCLNCX, pCLNRX, and pCLNDX) have an extended
packaging signal (Ψ +) and are derived from safetymodified
retrovirus vectors in which the gag open
reading frame has been stopped by a point mutation
(1), thereby minimizing the opportunity for replication
competent retrovirus production by recombination
with packaging genome.
The RetroMax system is designed for maximal virus
titer in 293 cells. It takes advantages of two properties
of 293 cells, i) high level of transfectibility, ii) strong
E1A-mediated stimulation of CMV promoter
controlled transcription. 293 cells are of nonmurine
origin, hence the problem of selective packaging and
transfer of VL30 genomes (present in all murine
packaging cells) are avoided. Vector supernatants are
free of helper virus and are of sufficiently high titer
within 2 days of transient transfection in 293 cells to
permit infection of more than 50% of dividing target
cells in culture.
Specifications/Features
Vector Size:
8.5 kb
Kit Contents:
10 µg in 20 µl 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)