Tyrosine Kinase Assay Kit, Colorimetric Detection
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EMD Millipore
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Product
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Tyrosine Kinase Assay Kit, Colorimetric Detection
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Company
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EMD Millipore
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Price
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More Information
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View Company Product Page
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Applications
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Kinase Assay
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Detection
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Colorimetric;Colorimetric
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Form
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Routinely evaluated by a reference peptide titration. Increasing amounts of Poly (Glu4-phospho-Tyr) Peptide, biotinylated were bound to streptavidin-coated wells. Total peptide mass/well was adjusted by the addition of Poly (Glu4-Tyr) Peptide, biotinylated to a well. Bound phospho-tyrosine containing peptide was detected with Anti-Phosphotyrosine, recombinant 4G10, HRP conjugate.
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Source/Expression System
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Enzymology kits or supplies
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Target
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Tyrosine Kinase
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Catalog Number
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17-315
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Method
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Colorimetric
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Original Item Name
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Tyrosine Kinase Assay Kit, Colorimetric Detection
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Quantity
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1 kit
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Description
Tyrosine Kinase Assay Kit, Colorimetric Detection This non-radioactive kinase assay kit is a two-stage peptide phosphorylation/colorimetric detection assay kit. In the first stage, a biotinylated substrate peptide containing tandem repeats of Poly (Glu4-Tyr) is incubated with a tyrosine kinase enzyme sample in the presence of non-radioactive ATP and a Mn2+/Mg2+ co-factor cocktail. The kinase assay can be performed with substrate peptide either in solution, or bound to the well of a streptavidin-coated 96-well plate. The second stage involves the detection of phosphorylated substrate by Direct Enzyme Linked Immunosorbent Assay (ELISA) using a monoclonal anti-phosphotyrosine-HRP (Horse Radish Peroxidase) antibody conjugate. Tetramethylbenzidine (TMB) is provided as the HRP substrate.
Specifications/Features
Streptavidin Coated Microwell Plate (Cat.# 20-183);Anti-pTyr, recombinant 4G10™, HRP;ELISA wash and blocking buffers;Poly (Glu4-Phospho-Tyr), biotinylated;Poly (Glu4-Tyr), biotinylated;Tyrosine Kinase Reaction Buffer, 5X;Sodium Orthovanadate, 50mM