Takara Taq Polymerase
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EMD Millipore
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Product
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Takara Taq Polymerase
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Company
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EMD Millipore
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Price
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More Information
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View Company Product Page
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Applications
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DNA Amplification
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Form
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10X PCR Reaction Buffer w/ optimized MgCl2 ; PCR test: Good performance of DNA amplification by PCR was confirmed by using lamda DNA as the template (amplified fragment: 8kbp). Good performance of DNA amplification of a single copy gene by PCR was also confirmed by using human placenta DNA as the template, and human p53-primers (amplified fragment: 2.9kbp).
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Original Item Name
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TaKaRa Taq™ Polymerase
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Source/Expression System
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Enzymes
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Catalog Number
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R001B
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Quantity
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1000 units
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Concentration
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5 U/μl
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Description
TaKaRa™ Taq is a recombinant, thermostable, 95 kDa DNA polymerase encoded by the DNA polymerase gene of the Thermus aquaticus YT-1 strain that has been cloned into Escherichia coli. It has been shown to have essentially the same characteristics as the native Taq DNA polymerase. The enzyme is supplied at a concentration of 5 units/µL with 10X PCR Reaction Buffer containing optimized MgCl2 and 2.5 mM dNTP Mix. Unit definition: One unit is the amount of the enzyme that will incorporate 10 nmol of dNTP into acid-insoluble products in 30 minutes at 74°C with activated salmon sperm DNA as the template-primer. Reaction mixture for unit definition: 25mM TAPs (pH9.3 at 25°C); 50mM KCl; 2mM MgCl2; 1mM 2-mercaptoethanol; 200µM each dATP, dGTP, dTTP; 100µM alpha P32-dCTP; 0.25mg/mL activated salmon sperm DNA