SlideHyb™ Glass Array Hybridization Buffer #1 (2 ml tube)
from
Ambion
Description
Ambion® SlideHyb™ Glass Array Hybridization Buffer #1 is the most basic of three available buffers and typically exhibits the lowest background of the three. The buffers vary in hybridization kinetics and blocking reagents, but have identical salt and formamide compositions, resulting in similar hybridization stringencies.
Many differences exist between the various commercial and lab-prepared glass arrays. The type of glass slide used, the length and GC content of the attached nucleic acid targets, and the manufacturing protocol employed all influence the optimal hybridization conditions for glass microarray analysis. The selection of SlideHyb™ Glass Array Hybridization Buffers allow you to determine the ideal conditions for your system.
When developing a glass array hybridization protocol, try multiple SlideHyb Buffers. Use SlideHyb Buffer #1 at various hybridization temperatures to determine the best temperature. Then, use that temperature to compare the various hybridization buffers. Choose the SlideHyb Buffer that gives the greatest signal and lowest background for your experiment.
Accessory Products:
Also available are SlideHyb™ Glass Array Hybridization Buffers #2 (SKU #AM8862) and #3 (SKU #AM8863). The Glass Array Hybridization Cassette (SKU #AM10040) is designed for glass microarray hybridization reactions.
Specifications/Features
Information:
The SlideHyb Survey Kit contains four unique SlideHyb Glass Array Hybridization Buffers and lets you select the conditions ideal for your glass array system.