NorthernMax™ Kit
from
Ambion
Description
The Ambion® NorthernMax® Kit contains a complete set of RNase-free reagents for running formaldehyde-based northern analysis. This kit is ideal for the investigator who wants to use familiar reagents while taking advantage of NorthernMax® Kit's high sensitivity. Sufficient reagents are provided to process 1000 cm2 membrane.
• Increase sensitivity up to 100x over standard hybridization protocols
• Reduce hybridization to just 2 hours for many messages
• Complete kit contains ULTRAhyb® Hybridization Buffer and gel and wash reagents for 10–20 gels
• Compatible with RNA, DNA, or oligonucleotide probes; labeled isotopically or nonisotopically
The included ULTRAhyb® Ultrasensitive Hybridization Solution increases the sensitivity of RNA probes up to 20x and DNA probes up to 100x. Hybridization can be completed in just 2 hours for many messages. The NorthernMax® Kit is compatible with DNA, RNA, or oligo probes labeled isotopically or nonisotopically. The NorthernMax® Kit contains everything you need to conduct northerns. Positively charged nylon membranes must be purchased separately (see Accessory Products). An RNA sample and template for probe synthesis are also provided as controls for each kit.
Accessory Products:
The BrightStar® -Plus Positively Charged Nylon Membranes (SKU# AM10100, AM10102, or AM10104) are optimized for use with the NorthernMax® Kit.
Specifications/Features
Information:
Each NorthernMax Kit provides a complete system. NorthernMax Kits contain everything you need to conduct Northerns, including high quality agarose, gel loading solution, gel buffer, running buffer, transfer buffer, ULTRAhyb Ultrasensitive Hybridization Buffer, wash solutions, and even RNaseZap, a nontoxic ribonuclease decontamination solution for equipment and work surfaces. Positively Charged Nylon Membranes must be purchased separately.