TRAPeze® ELISA Telomerase Detection Kit, strip well plates
from
EMD Millipore
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Product
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TRAPeze® ELISA Telomerase Detection Kit, strip well plates
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Company
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EMD Millipore
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Price
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More Information
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View Company Product Page
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Method
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ELISA
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Original Item Name
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TRAPeze® ELISA Telomerase Detection Kit, strip well plates
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Reactivity
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Mammals
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Source/Expression System
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Molecular biology test kits or supplies
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Target
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Telomerase
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Target/Molecule Descriptor
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TRAPEZE®
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Detection
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Request Info
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Catalog Number
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S7750
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Applications
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PCR
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Quantity
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96 assays
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Description
TRAPeze® ELISA Telomerase Detection Kit, strip well plates The TRAPeze® ELISA Telomerase Detection Kit is a rapid, non-isotopic assay for detecting telomerase activity in cell or tissue extracts. The method combines a one-step TRAP assay (PCR) with direct, chromogenic detection of TRAP products (ELISA). Recommended Taq polymerases: must be non-proofreading, having no exonuclease activity and capable of "hot-start." Titanium Taq™, Platinum® Taq are suggested. The kit uses coated strip-well pieces for ease of use and handling. Using this Manual The TRAPEZE® ELISA Telomerase Detection Kit provides reagents necessary to perform the Telomeric Repeat Amplification Protocol (TRAP) and ELISA assay for non-quantitative detection of telomerase activity in cells and tissues. The novice user is advised to read the entire manual prior to using the TRAPEZE® ELISA Kit, particularly Sec. III: Protocol. Supplemental protocols can be found in Sec. V. Appendix. Should additional questions arise, assistance can be obtained by contacting Chemicon Technical Support at (800) 437-7500 or at techserv@chemicon.com.
Specifications/Features
1. CHAPS XL Lysis Buffer (10 mL);2. 5X TRAPEZE® Reaction Mix (1 mL) • biotinylated TS primer • biotinylated RP primer •dA, dC, dG and dTTP •internal control primers •Tris buffer;3. PCR - Grade Water (10 mL) protease, DNase, and RNase-free; deionized;4. TSR8* (control template) (32 µL) 0.1 amole/µL TSR8 template;5. Control Cell Pellet Telomerase positive cells (106 cells);6. Anti-DNP Antibody, HRP-conjugated (22µL);7. Blocking/Dilution Buffer (45 mL);8. 10X Washing Buffer (45 mL);9. TMB Solution (10 mL) Tetramethylbenzidine (HRP substrate);10. Stop Solution (10 mL);11. Streptavidin-coated Microtiter Plate;* Caution - refer to Sec. II. Kit Components, Warning and Precautions.
TERT
(Telomere reverse transcriptase)