TRAPeze® XL Telomerase Detection Kit
from
EMD Millipore
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Product
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TRAPeze® XL Telomerase Detection Kit
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Company
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EMD Millipore
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Price
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More Information
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View Company Product Page
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Method
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Fluorometric
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Original Item Name
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TRAPeze® XL Telomerase Detection Kit
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Reactivity
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Mammals
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Sample Type
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cell and tissue extracts
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Source/Expression System
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Molecular biology test kits or supplies
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Target
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Telomerase
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Target/Molecule Descriptor
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TRAPeze®
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Detection
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Request Info
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Catalog Number
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S7707
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Applications
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PCR
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Quantity
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112 assays
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Description
TRAPeze® XL Telomerase Detection Kit The TRAPeze® XL Telomerase Detection Kit is a homogenous fluorescence assay for rapid and sensitive telomerase activity measurement in cell and tissue extracts. The TRAPeze® XL Kit incorporates the use of the novel Amplifluor™ fluorescence energy transfer-labeled primers into the original TRAPeze® Telomerase Detection Kit reaction so that quantitative measurements are obtained without radioactivity. Additionally, the unique design of Amplifluor primers enables homogeneous signal amplification and quantitation directly in the unopened PCR vessel. The closed-tube assay format eliminates the risk of contaminating unprocessed samples with PCR amplicon. The TRAPeze® XL Telomerase Detection Kit procedure does not require post-PCR manipulations such as gels or enzyme-linked immunosorbant assays (ELISAs). Recommended Taq polymerases: must be non-proofreading, having no exonuclease activity and capable of "hot-start." Titanium Taq™, Platinum® Taq are suggested.
Specifications/Features
CHAPS Lysis Buffer (13.5 mL);5X TRAPEZE® XL Reaction Mix (1.12 mL);TS primer;RP Amplifluor® primer;K2 Amplifluor® primer;TSK2 template;dA, dC, dG and dTTP;diluted in :;100 mM Tris-HCl, pH 8.3;7.5 mM MgCl2;315 mM KCl;0.25% Tween 20;5 mM EGTA;0.5 mg/mL BSA;PCR - Grade Water (8.2 mL);protease, DNase, and RNase-free;;deionized;TSR8* (control template) (45 µL);0.2 amole/µL TSR8 template;Control Cell Pellet;Telomerase positive cells (106 cells);* Caution - refer to Sec. II. Kit Components, Precautions.
TERT
(Telomere reverse transcriptase)