DESInducible/Secreted Kit with pCoHygro
from
Invitrogen
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Product
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DESInducible/Secreted Kit with pCoHygro
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Company
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Invitrogen
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Price
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Inquire
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More Information
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View Company Product Page
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Accession Number
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Request Info
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Applications
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Regulated, Secreted Expression in S2 Cells
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Conjugate/Tag/Label
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His ; V7
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Copy Number
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Host
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Insect
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Inducer
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Origin of Replication
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pMT/BiP/V5-His
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Promoter
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Recombination Site
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Reporter
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Resistance
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pCoHygro: Hygromycin resistance gene: bases 781-1830
pMT/BiP/V5-His: Ampicillin
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Selectable Marker
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Type
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3.6 kb
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Vector Name
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pMT/BiP/V5-His A, B, C
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Catalog Number
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K413001
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Quantity
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1set
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MCS
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Description
TubulinTracker™ Green reagent (T34075) provides greenfluorescent
staining of polymerized tubulin in live cells. Also
known as Oregon Green® 488 Taxol bis-acetate (a bi-acetylated
version of Oregon Green® 488 Taxol (P22310)), TubulinTracker™
Green reagent is an uncharged, nonfluorescent compound that
easily passes through the plasma membrane of live cells. Once
inside the cell, the lipophilic blocking group is cleaved by nonspecific
esterases, resulting in a green-fluorescent, charged form.
The non-acetylated version of Oregon Green® 488 Taxol has
previously been recommended for live-cell labeling; however,
TubulinTracker™ Green reagent permeates cells more readily,
providing more uniform labeling and better selectivity.
Specifications/Features
Vector Size:
3.6 kb
Kit Contents:
The DES® Inducible/Secreted Kits contains 20 µg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 µg each), GIBCO™ Schneider's Drosophila Medium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.
Features:
The pMT/BiP/V5-His vector offers the following additional features: