GeneTailor™ Site-Directed Mutagenesis System
from
Invitrogen
Description
The GeneTailor™ Site-Directed Mutagenesis System provides a simple, convenient, and highly efficient means to generate mutant target proteins. You can generate:
- All types of base substitutions, deletions, or insertions of up to 21 nucleotides
- Up to 96 different site-directed mutants from the same methylated plasmid
- Site-directed mutations with >80% mutagenesis efficiency
DNA isolated from any source can be used, with no need for specialized vectors, host strains, or restriction sites. A streamlined protocol provides >80% mutagenesis efficiency, saves time, and easily adapts to high, medium, and low throughput applications.
How the GeneTailor™ System Works
Mutagenesis with the GeneTailor™ System is a simple three-step process (Figure 1) requiring only three hours plus an overnight incubation following transformation. First, methylate target DNA (up to 8 kb, isolated from any bacterial source) with DNA methylase. Then perform the mutagenesis reaction, with two overlapping primers, only one of which must contain the desired mutation(s). Next, transform the mutated DNA. The methylated parental DNA is digested by the host endonuclease, leaving the unmethylated mutagenesis reaction product intact. No purification step or additional digestions are needed. Select for individual colonies the following day to verify the mutations.
Specifications/Features
# Rxns:
16 rxns