DNA PolI Klenow Fragment
from
New England Biolabs
Description
DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'→ 5' exonuclease activity, but has lost 5'→ 3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
Specifications/Features
Unit Definition:
One unit is defined as the amount of enzyme required to convert 10 nmol of dNTPs to an acid-insoluble form in 30 minutes at 37°C.