Description
Chemically competent Kluyveromyces lactis YCT598 cells are a suitable host for expressing proteins that have exhibited proteolysis in the parental GG799 background (NEB #C1001). YCT598 cells can be transformed with any linearized pKLAC-series or pKLMF-series expression vector. K. lactis strain YCT598 carries a selectable marker-free deletion of the KLLA0D15917g locus encoding an extracellular aspartyl protease that is homologous to S. cerevisiae Bar1p.