Description
Chemically competent Kluyveromyces lactis YCT389 (Δyps1) cells are a suitable host for expressing proteins that have exhibited proteolysis in the parental GG799 background (NEB #C1001). YCT389 cells can be transformed with any linearized pKLAC-series or pKLMF-series expression vector. K. lactis strain YCT389 carries a selectable marker-free deletion of the KLLA0E03938g locus encoding an extracellular aspartyl protease (KlYps1p) that is homologous to S. cerevisiae Yps1p.