Description
Chemically competent Kluyveromyces lactis YCT569 cells are a suitable host for expressing proteins that have exhibited proteolysis in the parental GG799 background (NEB #C1001). YCT569 cells can be transformed with any linearized pKLAC-series or pKLMF-series expression vector. K. lactis strain YCT569 carries a selectable marker-free deletion of the KLLA0D01507g locus encoding an extracellular aspartyl protease belonging to pfam00026.