Description
Chemically competent Kluyveromyces lactis YCT284 (Δcts1) cells are a preferred host for secreting heterologous proteins carrying a chitin binding domain tag. YCT284 cells can be transformed with any linearized pKLAC-series expression vector. K. lactis strain YCT284 carries a selectable marker-free deletion of the KLLA0C04730g locus encoding an extracellular chitinase (KlCts1p) which can degrade chitin chromatography resin and compete for resin binding sites (1).