Bac-to-Bac Baculovirus Expression System from Invitrogen


Product	Bac-to-Bac Baculovirus Expression System
Company	Invitrogen
Price	Pricing Info
More Information	View Company Product Page
Accession Number	Request Info
Applications	Baculovirus Expression
Copy Number	pFastBac™1: pUC origin, permits high-copy replication and maintenance in E. coli.
Host	Insect
Inducer	Request Info
MCS	Bam H I, Rsr II, BssH II, EcoR I, Stu I, Sal I, Sst I, Spe I, Not I, Nsp V, Xba I, Pst I, Xho I, Sph I, Kpn I, Hind III
Origin of Replication	f1, pUC
Promoter	Polyhedrin
Recombination Site	Request Info
Reporter	Request Info
Resistance	Ampicillin, Gentamicin
Selectable Marker	Ampicillin resistance gene Allows selection of the plasmid in E. coli. Gentamicin resistance gene Permits selection of the recombinant bacmid in DH10Bac™ E. coli.
Type	pFastBac™1: 4775 bp
Vector Name	PFastBac1 & pFastBac1-Gus
Catalog Number	10359016
Quantity	1 kit

Description

The Bac-to-Bac® Baculovirus Expression System is an efficient method for producing recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac® System relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells.

Specifications/Features

Kit Contents:
Each Bac-to-Bac® Baculovirus Expression System includes 10 µg of pFastBac™1 vector, 0.5 ml MAX Efficiency® DH10Bac™ Chemically Competent Cells, pFastBac™ 1-Gus control vector, and 1 ml of Cellfectin® Reagent. The Bac-to-Bac¤ Vector Kit contains only the expression and control vectors as described above. Store MAX Efficiency® DH10Bac™ Competent Cells at -80°C. Store Cellfectin® Reagent and vectors at +4°C. All components are guaranteed stable for 6 months when properly stored.

Features:
• Time-saving expression bacmid: With Bac-to-Bac®, the expression cassette of the pFastBac™ vector recombines with the parent bacmid in DH10Bac™ E. coli Competent Cells to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.

Vector Size:
pFastBac™1: 4775 bp