Description
Htatip, Htatip, Mouse GIPZ lentiviral shRNAmir individual clone
shRNAmir libraries for increased and specific knockdown
The Expression ArrestÖ microRNA-adapted shRNA (shRNAmir) libraries were developed in collaboration with Greg Hannon (CSHL) and Steve Elledge (Harvard). shRNAmir triggers for mammalian RNAi are based on current knowledge of the endogenous microRNA biogenesis pathway. shRNAmir constructs are designed to mimic a natural microRNA primary transcript, enabling specific processing by the endogenous RNAi pathway and producing more effective knockdown (Silva et al 2005). Genome wide shRNAmir libraries incorporate several features aimed at increasing the efficiency and specificity of gene knockdown providing solutions for diverse RNAi applications. These include:
- microRNA-30 adapted design for increased knockdown specificity and efficiency
- Transient, stable and in vivo options for RNAi
- Pre-cloned into retroviral and lentiviral vectors
- Human and mouse genomes targeted
- Guaranteed knockdown
GIPZ Lentiviral shRNAmir
shRNAmir-GFP: Visualize RNAi
- shRNAmir design for specificity and increased knockdown efficiency
- TurboGFP marks cells expressing shRNAmir
- Efficient low copy knockdown- Important for pooled screens
- Genome-wide human and mouse coverage
- Perform efficient RNAi in primary and non-dividing cells
- Guaranteed* knockdown
*When used in GIPZ RNAintro kits according to kit instructions.
HTATIP
(HIV 1 Tat interacting protein)