ER / PR IHC Kit
from
Invitrogen
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Product
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ER / PR IHC Kit
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Company
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Invitrogen
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Price
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Inquire
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More Information
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View Company Product Page
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Detection
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Request Info
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Method
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IHC
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Original Item Name
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ER/PR KIT
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Sample Type
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Tissue or cell preparations
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Target
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ER / PR
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Catalog Number
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95-6560
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Quantity
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1 Kit
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Description
Zymed’s HISTOSTAIN®-ER/PR KIT is designed to reveal the presence of Estrogen Receptor (ER) and Progesterone Receptor (PR) antigens in human tissue or cell preparations. Each stain requires a separate sample- this is not a double staining kit. These kits incorporate HorseRadish Peroxidase (HRP), streptavidin, and affinity-purified antibodies into the labeled-[strept]Avidin-Biotin (LAB-SA) method,(1,2) also known as Streptavidin-Biotin Amplification. After the primary antibody has been incubated with a sample, the LAB-SA method uses a biotinylated, affinity-purified secondary antibody to bind to the primary antibody. HRP labeled streptavidin is then bound to the biotinylated secondary antibody. The chromogen/substrate system (DAB for a brown signal) then creates an intense color deposit around the antigen/antibody/enzyme complex in the sample. The LAB-SA methodology is now widely used in basic research and routine histology labs.