Pierce Antibody Clean-up Kit
from
Thermo Scientific Pierce Protein Research Products
Description
The Thermo Scientific Pierce Antibody Clean-up Kit is a complete and easy-to-use kit for removing BSA and gelatin (up to 1%) from IgG samples. The Melon Gel IgG Purification Support included in this kit binds non-antibody proteins, such as BSA and gelatin, allowing the antibody to flow through in a purified form that is ready for storage and downstream applications. The Zeba Desalt Spin Columns contain an exclusive high-performance resin that offers exceptional desalting, ensuring that the IgG sample is in the optimal buffer for efficient purification.
Bovine serum albumin (BSA) and gelatin are commonly added to purified antibody solutions as stabilizers for long-term storage. Inclusion of 0.2-1.0% (2-10 mg/ml) BSA or gelatin helps stabilize antibody solutions that are 1 mg/ml, and generally does not interfere with immunodetection methods; however, protein additives, which are usually present at a concentration greater than the antibody, will interfere with most biotinylation, fluorescent dye labeling, antibody fragmentation and covalent immobilization methods. For such methods, the antibody must be isolated from the stabilizing proteins, and the Antibody Clean-up Kit accomplishes this efficiently.