Mouse IgG1 Fab and F(ab')2 Preparation Kit
from
Thermo Scientific Pierce Protein Research Products
Description
The Mouse IgG1 Fab and F(ab')2 Preparation Kits use immobilized ficin protease to generate Fab or F(ab')2 fragments from mouse IgG1. Both regular and micro-scale kits provide include all the reagents and accessories necessary to produce either Fab or F(ab')2 fragments depending on the concentration of cysteine that is used in the digestion reaction. When incubated with whole IgG1 and 1-4 mM cysteine, ficin produces F(ab')2 fragments. When 25 mM cysteine is used, ficin digestion produces Fab fragments.
The antibody digestion reaction is performed in convenient disposable spin columns that allow efficient removal of the immobilized protease and maximum recovery of the IgG fragments. Also included in the kits are Thermo Scientific NAb Protein A Spin columns and buffers to efficiently purify the resulting fragments. Protein A binds the Fc fragments and undigested IgG, allowing the pure Fab fragments to be recovered in the flow-through fraction. The kits also include Thermo Scientific Zeba Desalting Spin Columns for preparing the IgG sample quickly without dilution instead of utilizing time-consuming dialysis steps.