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Product
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AdEasy™ XL Adenoviral Vector System
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Company
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Agilent Technologies - Stratagene Products
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Price
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More Information
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View Company
Product Page
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Catalog Number
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240010
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Quantity
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1 kit
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Features
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N/C Term.
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Vector Name
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pShuttle vector, pShuttle-CMV vector, pShuttle-CMV-lacZ control vector
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Applications
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Recombinant adenoviruses are versatile tools for gene delivery and expression.
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Host
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mammalian
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Kit Contents
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Contains: 20 µg pShuttle vector, 20 µg pShuttle-CMV vector, 10 µg pShuttle-CMV-lacZ control vector, 5 x 0.1 ml BJ-5183-Ad1 electrocompetent cells, 10 µl Transformation control plasmid, 5 x 0.1 ml XL10-Gold® ultracompetent cells, 50 µl b-mercaptoethanol, 10 µl pUC18 DNA control plasmid, 1 x 106 Ad-293 cells
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MCS
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The vector pShuttle-CMV (provided with this kit) contains a multiple cloning site sandwiched between the CMV promoter and the SV40 polyadenylation signal and is suitable for insertion of a large cDNA (up to 6.6 kb). pShuttle (provided with this kit) conta
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Promoter
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pShuttle-CMV: CMV promoter
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Resistance
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pAdEasy-1 and the set of shuttle vectors contain different resistance cassettes: ampicillin and kanamycin, respectively.
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Selectable Marker
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Recombination Site
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Tags
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Tag Size
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Accession Number
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Reporter
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Copy Number
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Origin of Replication
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pBR322 origin of replication.
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Vector Size
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pAdEasy-1: 33.5 kb, pShuttle: 6.6 kb, pShuttle-CMV: 7.5 kb
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Vector Type
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pAdEasy-1: 33.5 kb, pShuttle: 6.6 kb, pShuttle-CMV: 7.5 kb
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Inducer
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