Description
The ELISA-Light™ immunoassay system is used for rapid, ultra-sensitive antigen detection in enzyme immunoassays employing an alkaline phosphatase label. The ELISA-Light™ system offers an excellent combination of high sensitivity and throughput for assays measured in a luminometer, such as the NorthStar™ HTS workstation or TR717™ microplate luminometer. The ELISA-Light™ system significantly extends the limits of detection of most immunoassays previously performed with colorimetric and isotopic methods. The kit contains reagents optimized for chemiluminescent ELISAs employing alkaline phosphatase labels and is available with a choice of ready-to-use chemiluminescent substrate formulations containing CSPD® or CDP-Star® substrate with Sapphire-II™ or Emerald-II™ luminescence enhancers. Multiple substrate/enhancer formulations provide options that let the user optimize specific assays or luminometers. The specific formulation that will provide the highest sensitivity for a specific assay can vary. Therefore, an ELISA sampler kit containing all four substrate/enhancer formulations is available to facilitate assay development.