Change-IT Multiple Mutation Site Directed Mutagenesis Kit
from
Affymetrix / USB
Description
The Change-IT™ Multiple Mutation Site Directed Mutagenesis Kit is designed to create single or multiple oligonucleotide-directed sequence changes in plasmids.
- Based on highly efficient exponential amplification using non-overlapping primers; eliminates primer-dimer formation
- Create single or multiple mutations in plasmids
- Create mutations in plasmids as large as 15 Kb
- Create insertions, base changes, or deletions
- Create deletions as large as 300 bp
- Encode multiple base changes in a single primer
- Three step process: amplification, DpnI digestion, and transformation
- Single day method
Specifications/Features
Advantages:
*Eliminates primer-dimer formation
*Create single or multiple mutations in plasmids
*Create mutations in plasmids as large as 15 Kb
*Create insertions, base changes, or deletions
*Create deletions as large as 300 bp
*Encode multiple base changes in a single primer
*Single day method
Mutation Rate:
Exponential amplification of the mutated plasmid provides for high mutagenesis efficiency, even with multiple mutations.