HotStart-IT FideliTaq PCR Master Mix (2X)
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Affymetrix / USB
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Product
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HotStart-IT FideliTaq PCR Master Mix (2X)
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Company
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Affymetrix / USB
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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High-specificity and high-sensitivity PCR amplificationExtremely long PCR amplificationPCR-mediated cloningHigh throughput PCR
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Original Item Name
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HotStart-IT FideliTaq PCR Master Mix (2X)
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Source/Expression System
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E. coli strain expressing a full length, unmodified clone of Taq DNA Polymerase from Thermus aquaticus(1-3).
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Catalog Number
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71156 500 RX
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Quantity
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500 reactions
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Description
HotStart-IT® FideliTaq™ Master Mix combines a novel hot start method developed at USB with the long and accurate amplification properties of FideliTaq DNA Polymerase. The new hot start method, called primer sequestration, uses a binding protein to reduce or eliminate nonspecific primer-extension products which may be generated at lower temperatures during assembly of PCR reactions. Following the initial denaturation step during PCR, the binding protein is inactivated and the primers are free to participate in the amplification reaction. This novel hot start method enhances many complex PCR reactions by increasing specificity and yield (Fig. 1) as well as sensitivity (Fig. 2).
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| Fig. 1. Increased specificity of the HotStart-IT® FideliTaq™ PCR Master Mix and stability. A 306 bp fragment of the single-copy numb gene was amplified from 1 ng of human genomic DNA with and without USB HotStart-IT technology. The primers in this assay were designed with 3 bases of overlap at their 3-ends to favor primer-dimer formation during reaction set-up at room temperature. Results demonstrate a shift from mainly primer-dimers to the desired product when HotStart-IT is used. Also, the mix is extremely stable as no loss in performance was observed following 10 freeze-thaw cycles (FTs) relative to a mix stored at -20°C. |
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| Fig. 2. Sensitivity of the HotStart-IT® FideliTaq™ PCR Master Mix. A 455 bp fragment of the single-copy numb gene was amplified from the indicated amounts of human genomic DNA. The master mix is extremely sensitive as amplification can be achieved from approximately one human cell. |
HotStart-IT FideliTaq Master Mix combines HotStart-IT FideliTaq DNA Polymerase, and Ultrapure nucleotides in a proprietary reaction buffer. Simply add template, primers, and water and the reactions are ready for cycling. The mix increases amplification fidelity up to 6-fold over Taq DNA Polymerase alone and generates much longer PCR products(1-4) (Fig. 3). PCR products have ends which are compatible with either blunt-end or TA cloning procedures(5) with A-tailed ends favored over blunt ends in an approximately 3 to 1 ratio.
Specifications/Features
Information:
Components:
HotStart-IT FideliTaq Master Mix (2X)
25 reactions: 625 µl
100 reactions: 4 x 625 µl
500 reactions: 12.5 ml
Note: Biocompare disclaims any information on this site.
Price information is approximate list price and actual prices may vary.
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